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Amir Arastehfar successfully defended his thesis by Webmaster News 2019-12-10 07:21:28
 

On December 9, 2019 Amir Arastehfar successfully defended his thesis ‘Development of Fungal Identification Tools and Evaluation of Microbiological and Clinical Profiles of Candida Species from Iran.’
Promotor Teun Boekhout appreciates the hard work and the high level of Dr. Arastehfar’s research. The main aim of his research: developing reliable and relatively low-cost identification tools for clinically important yeasts. Especially in developing countries identification of fungal infections is a challenge, since the best working diagnostic tools are expensive and time consuming. More affordable techniques are mostly time consuming and inaccurate, leaving a lot to be desired.
Amir Arastehfar developed ‘YEAST PANEL” multiplex PCR which succesfully identifies 21 target species in three multiplex PCR assays. Those 21 species are responsible for 95% of all yeast driven infections in Iran. Besides promising being cheap and accurate the new technique saves precious time. The turn-around is a few hours compared to the 48-72 hours of currently used diagnostic tools.

 




Amir Arastehfar, selection of his publications (2016-2019)

  1. Amir Arastehfar, Farnaz Daneshnia, Ahmed Hafez, Sadegh Khodavaisy, Mohammad-Javad Najafzadeh, Arezoo Charsizadeh, Hossein Zarrinfar, Mohammadreza Salehi, Zahra Zare Shahrabadi, Elahe Sassani, Kamiar Zomorodian, Weihua Pan, Ferry Hagen, Macit Ilkit, Markus Kostrzewa, and Teun Boekhout (2019). Antifungal susceptibility, genotyping, resistance mechanism, and clinical profile of Candida tropicalis blood isolates. Medical Mycology, In press.
  2. A. Arastehfar, BL. Wickes, M. Ilkit, DH. Pincus, F. Daneshnia, W. Fang, W. Pan, T. Boekhout. (2019) Identification of mycoses in developing countries. Journal of Fungi. 5, 90. doi: 10.3390/jof5040090. https://www.ncbi.nlm.nih.gov/pubmed/31569472
  3. A. Arastehfar, W. Lim, F. Daneshnia, W. van de Sande, AH. Fahal, M. Desnos-Ollivier, G.S. de Hoog, Teun Boekhout, Sarah. A. Ahmed. (2019) Madurella real-time PCR, a novel approach for eumycetoma diagnosis. PLOS Neglected Tropical Diseases. In press.
  4. A. Arastehfar, W. Fang, W. Pan, M. Lackner, W. Liao, P. Badiee, K. Zomorodian, H. Badali, F. Hagen, C. Lass-Flörl and T. Boekhout. (2018) YEAST PANEL Multiplex PCR for identification of yeast pathogens: stepwise diagnostic strategy, useful for developing countries. Diagnostic Microbiology and Infectious Diseases. 93(2):112-119. doi: 10.1016/j. diagmicrobio.2018.09.007. https://www.sciencedirect.com/science/article/pii/S073288931830381X
  5. A. Aarstehfar, F. Daneshnia, M. Kord, M. Roudbary, H. Zarrinfar, W. Fang, SJ. Hashemi, MJ. Najafzadeh, S. Khodavaisy, W. Pan, H. Badali, W. Liao, S. Rezaie, T. Boekhout. (2018) Comparison of 21-plex PCR and API 20C AUX, MALDI-TOF MS, and rDNA sequencing for a wide range of clinically isolated yeast species: Improved identification by combining 21-plex PCR and API 20C AUX as an alternative strategy for developing countries. Frontiers in Cellular and Infection Microbiology. 9:176. doi: 10.3389/fcimb.2019.00176. https://www.frontiersin.org/articles/10.3389/fcimb.2019.00021/full
  6. A. Arastehfar, W. Fang, H. Badali, A. Vaezi, W. Jiang, W. Pan, F. Hagen, T. Boekhout. (2018) Low-Cost Tetraplex PCR for the Global Spreading Multi-Drug Resistant Fungus, Candida auris and Its Phylogenetic Relatives. Frontiers in Microbiology. 29; 9:1119. doi: 10.3389/fmicb.2018.01119. https://www.ncbi.nlm.nih.gov/pubmed/29896181
  7. A. Arastehfar, W. Fang, F. Daneshnia, A. AM. Al-Hatmi, W. Liao, W. Pan, Z. Khan, S. Ahmad, K. Rosam, M. Lackner, C. Lass-Florl, F. Hagen, T. Boekhout, (2018) Novel multiplex qPCR approach for direct detection of Candida auris and its relatives in spiked serum samples. Future Microbiology. 4:33-45. doi: 10.2217/fmb-2018-0227. https://www.ncbi.nlm.nih.gov/pubmed/30539665
  8. A. Arastehfar, F. Daneshnia, S. Farahyar, W. Fang, M. Salimi, MR. Salehi, F. Hagen, W. Pan, M. Roudbary, T. Boekhout. (2019) Incidence and spectrum of yeast species isolated from the oral cavity of Iranian patients suffering from hematological malignancies. Journal of Oral Microbiology. 11(1):1601061. doi: 10.1080/20002297.2019.1601061. https://www.tandfonline.com/doi/full/10.1080/20002297.2019.1601061.
  9. A. Arastehfar, F. Daneshnia, MR. Salehi, H. Zarrinfar, S. Khodavaisy, PJ. Haas, M. Roudbary, MJ. Najafzadeh, K. Zomorodian, A. Charsizadeh, C. Brouwer, W. Pan, F. Hagen, T. Boekhout. (2019) Molecular characterization and antifungal susceptibility testing of Candida nivariensis from blood samples – an Iranian multicenter study and a review of the literature. Journal of Medical Microbiology. 68(5):770-777. doi: 10.1099/jmm.0.000963. https://www.ncbi.nlm.nih.gov/pubmed/30924763.
  10. A. Arastehfar, S. Khodavaisy, F. Daneshnia, MJ. Najafzadeh, S. Mahmoudi, A. Charsizadeh, MR Salehi, Hossein Zarrinfar, A. Raeisabadi, S. Dolatabadi, Z. Zare Shahrabadi, K. Zomorodian, W. Pan, F. Hagen, T. Boekhout. (2019) Molecular identification, genotypic diversity, antifungal susceptibility, and clinical outcomes of infections caused by clinically underrated yeasts, Candida orthopsilosis and Candida metapsilosis: An Iranian multicenter study (2014-2019). Frontiers in Cellular and Infection Microbiology. 9:264. doi: 10.3389/ fcimb.2019.00264. https://www.ncbi.nlm.nih.gov/pubmed/31417877.