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Keywords:GENBANK/AB013502 GENBANK/AB013503 GENBANK/AB013504 GENBANK/AB013505 GENBANK/AB013506 GENBANK/AB013507 GENBANK/AB013508 GENBANK/AB013509 GENBANK/AB013510 GENBANK/AB013511 GENBANK/AB013512 GENBANK/AB013513 GENBANK/AB013514 GENBANK/AB013515 GENBANK/AB013516 GENBANK/AB013517 GENBANK/AB013518 GENBANK/AB013519 GENBANK/AB013520 GENBANK/AB013521 GENBANK/AB013522 GENBANK/AB013523 GENBANK/AB013524 GENBANK/AB013525 GENBANK/AB013526 GENBANK/AB013527 GENBANK/AB013528 GENBANK/AB013529 GENBANK/AB013530 GENBANK/AB013531 Candida/*classification/genetics *Codon DNA, Ribosomal/chemistry Phylogeny RNA, Ribosomal, 18S/genetics Translation, Genetic 
Abstract:CUG, a universal leucine codon, was reported to be read as serine in 10 species of the genus Candida. We used an in vitro cell-free translation system to identify the amino acid assignment of codon CUG in 78 species and 7 varieties of galactose-lacking Candida species equipped with Q9 as the major ubiquinone. Of these, only 11 species used codon CUG as a leucine codon. The remaining species decoded CUG as serine. Their small subunit ribosomal DNA sequences were also determined and analyzed using both Neighbor-Joining and Maximum Likelihood methods. The species decoding CUG as serine and leucine formed distinct clusters on both molecular phylogenetic trees. Our result suggests that non-universal decoding is not a rare event, and that it is widely distributed in the genus Candida. 
Summary:Sugita,T.;Nakase,T. 1999. Systematic and Applied Microbiology. 22 
Document Reference #:9601